protocols  

  CellSeedingProtocol   protocol1   protocol3   protocol3  
 

Preparing nanofibers for cell culture

 

 

 

 

Preparation of agar plates to make aggregates of adherent cells

 

Preparation of aggregates for adherent cells

 

Seeding Cell aggregates on nanofiber plates

 
  cell fix   protocol4   protocol4   protocol4  
 

Preparation of agar plates to make cell spheroids

 

 

 

 

Preparation and culture of cell spheroids on nanofibers

 

Staining and seeding dissociated adherent cells on nanofiber plates

 

Seeding giloblastoma neurospheres or dissociated giloblastoma stem cells

 
  CellSeedingProtocol   protocol1   protocol3   protocol3  
 

Measurement of cell viability on nanofibers

 

 

 

 

Measurement of Cell Toxicity on nanofibers

 

Measuring axial cell dispersion on aligned nanofibers

 

Immunocytochemistry of cells on nanofibers

 
  cell fix   protocol4          
 

Cell Recovery from nanofibers

 

 

 

 

Fixation Procedure for SEM (cells on nanofiber scaffolds)